Production of Lipase from Pseudozyma aphidis and Determination of the Activity and Stability of the Crude Lipase Preparation in Polar Organic Solvents

Dimitrijevic, Aleksandra S and Velickovic, Dusan V and Bezbradica, Dejan I and Bihelovic, Filip J and Jankov, Ratko M and Milosavic, Nenad B (2011) Production of Lipase from Pseudozyma aphidis and Determination of the Activity and Stability of the Crude Lipase Preparation in Polar Organic Solvents. Journal of the Serbian Chemical Society, 76 (8). p. 1081. ISSN 0352-5139 (FP7- 256716)

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Abstract

The production of lipase from Pseudozyma aphidis (DSM 70725) was determined in six different media. The highest lipase production was observed in a medium with glucose as the sole carbon source, and yeast extract and sodium nitrate as the nitrogen sources. The time course studies of growth and lipase production in the optimal medium revealed that the highest lipase production was achieved at the end of the log phase of growth, reaching the value of 35.0 U cm-3 in the fifth day of cultivation. The effects of various polar, water-miscible, organic solvents on the activity and stability of the crude lipase produced by P. aphidis were evaluated. The hydrolytic activity of the crude lipase towards p-nitrophenyl palmitate (p-NPP) in aqueous media and in organic solvents was determined, using the same spectrophotometric assay in both the aqueous and organic media. The crude lipase preparation exhibited activity towards p-NPP only in acetone and acetonitrile, while the lipase was stable only in acetone, with 23 % residual activity after 24 h of incubation. These results suggested that lipase from P. aphidis can be used as a biocatalyst for potential applications in such organic solvents.

Keywords

lipase; activity; stability; organic solvents

Item Type:	Article
FP7 Grant Agreement Number:	256716
FrameWork Programmes:	SP4-Capacities
Scientific Areas:	Research Potential
Contact Email Address:	nenadmil@chem.bg.ac.rs
Last Modified:	02 Aug 2012 09:22
Access rights:	Open access
URI:	http://eprints.kobson.nb.rs/id/eprint/6

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